More slides

diff --git a/antismash_lca2011.html b/antismash_lca2011.html
index 3bb44ff2188c712dd32af050fdf648e5bfb8037a..867a083f751b4aed65b1ba4c278ea8df3cf83979 100644 (file)
--- a/antismash_lca2011.html
+++ b/antismash_lca2011.html
@@ -191,6 +191,7 @@
 
   <section class="slide" id="background-nrps">
   <h2>Non-Ribosomal Peptide Synthesis</h2>
+  <img src="nrps.svg">
   </section>
 
   <section class="slide" id="main-talk">
@@ -212,6 +213,8 @@
   </ul>
   </section>
 
+
+
   <section class="slide" id="thanks">
   <h1>Thank you</h1>
   </section>

diff --git a/drawings/nrps.odg b/drawings/nrps.odg
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diff --git a/drawings/server_architecture.odg b/drawings/server_architecture.odg
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diff --git a/nrps.svg b/nrps.svg
new file mode 100644 (file)
index 0000000..63d8384
--- /dev/null
+++ b/nrps.svg
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diff --git a/script.txt b/script.txt
index 1f3374cd1afcc84f9c2bc332cc497984584d08b3..870dca93c79ac836da3ac93afa171b96b805e496 100644 (file)
--- a/script.txt
+++ b/script.txt
@@ -220,6 +220,38 @@ For example, I was able to buy this tube of triple-antibiotic ointment at
 Wallmart for less than three dollars. If I misused this, I'd have a good shot
 at creating bacteria resistant to three different antibiotics.
 
+Remember how the central dogma of molecular biology went? From DNA to mRNA to
+protein. Now let me show you one of the exceptions I was talking about earlier.
+Some bacteria and moulds have a completely different way of building proteins.
+There is no blueprint for the product, no mRNA involved and the ribosome never
+sees anything in the process. Instead, the cell builds a huge megaenzyme that
+works just like a factory production line. Many different modules perform a
+well-defined reaction. Then they get the next piece of work where they perform
+the exact same reaction again, rinse, repeat.
 
+So why the heck does the cell bother with a whole new way of producing
+proteins? First of all, compared to the proteins produced by a ribosome, the
+factory-made proteins can contain unusual building blocks. The ribosome is a
+multipurpose machine that can deal with 20 amino acids without requiring any
+changes. A module in the production line megaenzyme is specialized on dealing
+with a single amino acid, but can be designed to deal with non-standard
+amino acids as well.
+
+Also, the production line approach can produce a much higher amount of product
+per timeframe. While the ribosome is building up products at one step at a
+time, the production line performs all the steps at every cycle. So using a
+production line megaenzyme, the cell can pump out a lot of product really fast.
+Because this system allows the cell to build peptides without involving a
+ribosome, this is called non-ribosomal peptide synthesis. The megaenzyme is a
+non-ribosomal peptide synthase, or NRPS in short.
+
+With the biological background part out of the way, let's talk about how I'm
+using antiSMASH to identify gene clusters involved in the production of
+antibiotics. Remember the biology part, I'll be handing out a graded test at
+the end of the talk. Sorry, giving talks in university lecture hall triggers
+teaching reflexes.
+
+antiSMASH, the antibiotics and secondary metabolites analysis shell, is a
+modular pipeline that uses a host of exsiting bioinformatics tools to search
+genomes for secondary metabolite gene clusters.
 
-(Quip about a graded test at the end of the talk)

diff --git a/server_architecture_old.png b/server_architecture_old.png
index 726f9149c603da92fc8a892fcea846feaab39de3..0ab1c0a5374f555a12d1df29ae4b79f1b9d79c4a 100644 (file)
Binary files a/server_architecture_old.png and b/server_architecture_old.png differ